Stable cell line development is the exogenous gene enters the recipient cell and integrates into the cell chromosome, so that the host cells can stably express the target gene in the long term. Stable cell lines are widely used in the fields of recombinant protein, antibody production, gene editing, functional studies and other fields. ClongeneBio has been engaged in protein antibody expression research for many years, has a large amount of experience in the development of stable cell lines, and can provide diversified services to meet the needs of customers.
- Codon optimization and gene synthesis
- Subcloning into expression plasmids
- Plasmid amplification and preparation
- Transient expression
- Expression level detection by ELISA or WB
- Stable transfection
- Stable Pool selection
- Subcloning
- Single clone selection and screening
- 100+ mg
- QC analysis
Gene synthesis
Vector construction
Transient expression evaluation
Stable cell line generation selection and screening
Single clone selection and screening
Protein expression and purification
Product delivery
Customer Consultation
Program and contract confirmation
Project initiation
Feedback and sample delivery
Scalable production
Product delivery
Case #1. Expression of the HP mAb in the constructed CHO-K1 stable cell line
ClongeneBio constructed the CHO-K1 stable cell line, which can express the HP mAb. The affinity test result after expression and purification is shown as Figure 1.
